子宫内膜腺上皮及基质细胞分离、培养作为子宫内膜异位症体外细胞模型的探索The exploration of the isolation and culture of eutopic endometrial glandular epithelial cell and its stromal cell as an in vitro model of endometriosis.
谭先杰,刘东远,郎景和,沈铿,冷金花,孙大为,朱兰,刘珠凤,许秀英
摘要(Abstract)：
目的 :探索在位子宫内膜腺上皮及基质细胞的分离、培养作为子宫内膜异位症 (EMs)的体外细胞模型。方法 :通过酶解、系列过滤、沉降及贴壁纯化等技术 ,分离、纯化及培养 15份EMs患者的在位子宫内膜腺上皮细胞及其基质细胞 ,并拟传代。结果 :11份标本获得成功 ;每 1g新鲜子宫内膜组织可得到 (8～ 12 )× 10 6个原代基质细胞及 (4～ 8)× 10 6个原代腺上皮细胞 ;基质细胞纯度率可达 95 %以上 ,腺上皮细胞的纯度率约为90 %。基质细胞可以有限的传代 ,腺上皮细胞不能传代。通过改良步骤 ,可提高基质细胞的产量。结论 :在位子宫内膜腺上皮及其基质细胞的分离、培养可作为EMs的体外细胞模型之一。
关键词(KeyWords)： 子宫内膜异位症;子宫内膜;上皮细胞;基质细胞;体外细胞模型
基金项目(Foundation): 国家自然科学基金资助课题 (39830 35 0 )
作者(Author): 谭先杰,刘东远,郎景和,沈铿,冷金花,孙大为,朱兰,刘珠凤,许秀英
DOI: 10.13283/j.cnki.xdfckjz.2002.01.013
参考文献(References)：
- [1] SugawaraJ,YoshidaH ,FukayaT ,etal.Increasedsecretionofhepatocytegrowthfactorbyeutopicendometrialstromalcellsinwomenwithendometriosis[J].FertilSteril,1997,68:468472
- [2] OventonCE ,BarolwDH ,FernandezShawS ,etal.Invitrocultureofendometrialstormalandglandcellsasamodelforendometriosis:Theeffectofperitonealfluidonproliferation[J].FertilSteril,1997,67:5156
- [3] SharpeKL ,ZimmerRL ,KhanRS ,etal.Proliferativeandmor phogenicchangesinducedbythecocultureofratuterineandperitonealcells:Acellculturemodelforendometriosis[J].FertilSteril,1992,58:12201229
- [4] AriciA ,SenturkLM ,SeliE ,etal.Regulationofmonocytechemotacticprotein1expressioninhumanendometrialstro malcellsbyestrogenandprogesterone[J].BiolReprod,1999,61:8590
- [5] RyanIP ,SchriockED ,TaylorRN .Isolation,characterization,andcomparisonofhumanendometrialandendometriosiscellsinvitro[J].JClinEndocriolMetab,1994,78:642649
- [6] SharpeKL ,ZimmerRL ,GriffinWT ,etal.Polypeptidessyn thesizedandreleasedbyhumanendometriosistissuedifferfromthoseoftheuterineendometriuminculture[J].FertilSteril,1993,60:839851
- [7] SharpeTimmsK .Basicresearchesinendometriosis[J].Ob stetGynecolClinofNorthAmerica,1997,242:296290
- [8] MatthwsGJ ,RedfernCPF ,HirstBH ,etal.Characterizationofhumanpurifiedandstromalcellsfromendometriumanden dometriosisintissueculture[J].FertilSteril,1992,57:990997