M-MSNs作为基因载体应用于卵巢癌的可行性研究The feasibility study of M-MSNs used as gene carrier in ovarian cancer
王欣然;陈一杰;王云飞;刘婷;古宏晨;狄文;
摘要(Abstract)：
目的:研究磁性介孔二氧化硅纳米粒(M-MSN)作为小干扰RNA(siRNA)载体应用于卵巢癌的可行性,并初步探讨其作用机制。方法:CCK-8试剂盒检测M-MSN对SKOV3的细胞毒作用,共聚焦显微镜下观察SKOV3细胞对M-MSN的摄取以及M-MSN的溶酶体逃逸并释放siRNA的过程。磁共振成像(MRI)观察纳米粒对原位SKOV3肿瘤的靶向性。结果:与M-MSN共培养24h后,SKOV3细胞的生长未受影响。与细胞共培养后,M-MSN颗粒可被SKOV3细胞大量摄取,保护siRNA免被溶酶体内的水解酶溶解,并在细胞内与功能完整的siRNA成功分离。SKOV3原位荷瘤体内实验发现,静脉注射MMSN纳米粒后,M-MSN可成功到达卵巢原位,并在肿瘤组织中积聚。结论:M-MSN是siRNA的良好载体,具有很好的生物安全性,可成功保护siRNA免被破坏,并释放到卵巢癌细胞质内。同时由于M-MSN颗粒的T2负增强造影剂作用,可同时实现肿瘤部位显像,其作为新型诊疗一体纳米颗粒具有极大的应用价值。
关键词(KeyWords)： 卵巢癌;小干扰RNA;介孔无机颗粒;磁共振成像
基金项目(Foundation): 卵巢癌物理治疗临床转化研究示范基地(No:11DZ2211002);; 上海市科委纳米专项基金(No:11nm0503000);; 上海市高等学校科学技术发展基金(No:zzjdyx13060)
作者(Authors): 王欣然;陈一杰;王云飞;刘婷;古宏晨;狄文;
DOI: 10.13283/j.cnki.xdfckjz.2014.07.006
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