CXCR7及其配体在子宫内膜癌中的表达与意义Expression and significance of CXCR7 chemokine receptor and its ligands in endometrial carcinoma
顾红琴;贺银燕;赵贵旭;赖允丽;王倩倩;孙姗姗;席晓薇;张箴波;丰有吉;
摘要(Abstract)：
目的:检测趋化因子受体CXCR7(chemokine receptor 7)及其配体在子宫内膜癌组织和子宫内膜癌细胞中的表达,并研究雌激素对该受体的调控作用。方法:首先通过RT-PCR分析20种趋化因子受体在子宫内膜癌细胞株Ishikawa(ERα阳性)和AN3CA(ERα阴性)的转录水平,筛选出子宫内膜癌细胞株高表达的趋化因子受体,以免疫细胞化学染色法测定该受体及其相应配体在子宫内膜癌组织和细胞中的表达,并用蛋白质印迹法观察雌激素对该受体蛋白水平表达的影响。结果:(1)CXCR7 mRNA在子宫内膜癌细胞株Ishikawa、AN3CA均呈高表达,免疫细胞化学示子宫内膜癌组织和Ishikawa、AN3CA细胞表达CXCR7及其配体SDF-1,不表达I-TAC;(2)在Ishikawa中,雌激素在一定浓度范围内上调细胞中CXCR7蛋白水平的表达,而在AN3CA中,雌激素对CXCR7蛋白并无明显的调节作用。结论:CXCR7/SDF-1高表达于子宫内膜癌组织和子宫内膜癌细胞中,且雌激素上调CXCR7的表达,表明CXCR7/SDF-1可能在子宫内膜癌中发挥调控作用。
关键词(KeyWords)： 子宫内膜癌;雌激素;趋化因子受体;CXCR7;SDF-1;I-TAC
基金项目(Foundation): 国家自然科学基金青年项目(No:81001154/H1621);; 上海市科委青年科技启明星计划(No:11QA1405300)
作者(Authors): 顾红琴;贺银燕;赵贵旭;赖允丽;王倩倩;孙姗姗;席晓薇;张箴波;丰有吉;
DOI: 10.13283/j.cnki.xdfckjz.2012.07.023
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