中心体蛋白Nlp对卵巢癌细胞侵袭与黏附能力的影响Effects of Nlp on invasiveness and adherence of ovarian cancer cells
曲丹妮;李媛;张震宇;詹启敏;
摘要(Abstract)：
目的:探讨中心体蛋白Nlp是否促进卵巢癌细胞增殖、黏附与侵袭。方法:利用转染技术构建Nlp高表达的卵巢癌SKOV3细胞系,采用MTT法、肿瘤细胞黏附、跨膜迁移分析和平板集落形成率检测,比较SKOV3、SKOV3/GFP和SKOV3/Nlp细胞系在增殖、黏附与侵袭能力方面的差异。结果:与对照细胞相比,SKOV3/Nlp细胞的生长加快,SKOV3/Nlp细胞克隆形成率显著上升(P<0.05)。SKOV3/Nlp细胞在30min及60min时黏附率均显著大于SKOV3/GFP及SKOV3细胞(P<0.01)。SKOV3/Nlp、SKOV3/GFP、SKOV3细胞迁移至膜下的数目分别为:640±69,316±25,314±37。与SKOV3/GFP、SK-OV3细胞相比,SKOV3/Nlp细胞的迁移能力显著增强(P<0.05)。结论:当Nlp表达增高后,会导致肿瘤细胞的增殖、黏附、侵袭等恶性表型增加,提示Nlp具有促进肿瘤发生发展的生物学作用。
关键词(KeyWords)： Nlp;卵巢肿瘤;中心体异常;肿瘤侵润;肿瘤转移;细胞系,SKOV3
基金项目(Foundation): 973国家重点基础研究发展规划项目基金(No:2002CB513101);; 国家自然科学基金(No:30730046)
作者(Authors): 曲丹妮;李媛;张震宇;詹启敏;
DOI: 10.13283/j.cnki.xdfckjz.2013.02.005
参考文献(References)：
- [1]Pihan GA,Wallace J,Zhou Y,et al.Centrosome abnormal-ities and chromosome instability occur together in pre-in-vasive carcinomas[J].Cancer Res,2003,63(6):1398-1404
- [2]Pihan GA,Purohit A,Wallace J,et al.Centrosome defectscan account for cellular and genetic changes that charac-terize prostate cancer progression[J].Cancer Res,2001,61(5):2212-2219
- [3]Balczon R,Varden CE,Schroer TA.Role for microtubulesin centrosome doubling in Chinese hamster ovary cells[J].Cell Motil Cytoskeleton,1999,42(1):60-72
- [4]Chiba S,Okuda M,Mussman JG,et al.Genomic conver-gence and suppression of centrosome hyperamplification inprimary p53-/-cells in prolonged culture[J].Exp CellRes,2000,258(2):310-321
- [5]D'Assoro AB,Lingle WL,Salisbury JL.Centrosome ampli-fication and the development of cancer[J].Oncogene,2002,21(40):6146-6153
- [6]Duensing S.A tentative classification of centrosome abnor-malities in cancer[J].Cell Biol Int,2005,29(5):352-359
- [7]赵雪莲,宋咏梅,金顺钱,等.Cdc2/cyclin B1对中心体蛋白Nlp的调控在细胞生长中的作用[J].医学研究杂志,2010,39(12):25-29
- [8]Wang Y,Zhan Q.Cell cycle-dependent expression of cen-trosomal ninein-like protein in human cells is regulated bythe anaphase-promoting complex[J].J Biol Chem,2007,282(24):17712-17719
- [9]尹宁,童彤,詹启敏,等.应用组织芯片技术研究Bacp/Nlp在食管鳞癌中的表达及意义[J].中国组织化学与细胞化学杂志,2009,18(4):476
- [10]Yu L,Song Y,Zhang Q,et al.Ninein-like protein is over-expressed in head and neck squamous cell carcinoma andcontributes to cancer growth and resistance to apoptosis[J].Oncol Rep,2009,22(4):789-798
- [11]马莹,白萍,宋咏梅,等.中心体蛋白Nlp在宫颈癌中的表达及意义[J].肿瘤学杂志,2011,17(2):90-92
- [12]Qu D,Qu H,Fu M,et al.Increased expression of Nlp,apotential oncogene in ovarian cancer,and its implicationin carcinogenesis[J].Gynecol Oncol,2008,110(2):230-236