中心体蛋白Nlp对卵巢癌细胞侵袭与黏附能力的影响Effects of Nlp on invasiveness and adherence of ovarian cancer cells
曲丹妮;李媛;张震宇;詹启敏;
摘要(Abstract)：
目的:探讨中心体蛋白Nlp是否促进卵巢癌细胞增殖、黏附与侵袭。方法:利用转染技术构建Nlp高表达的卵巢癌SKOV3细胞系,采用MTT法、肿瘤细胞黏附、跨膜迁移分析和平板集落形成率检测,比较SKOV3、SKOV3/GFP和SKOV3/Nlp细胞系在增殖、黏附与侵袭能力方面的差异。结果:与对照细胞相比,SKOV3/Nlp细胞的生长加快,SKOV3/Nlp细胞克隆形成率显著上升(P<0.05)。SKOV3/Nlp细胞在30min及60min时黏附率均显著大于SKOV3/GFP及SKOV3细胞(P<0.01)。SKOV3/Nlp、SKOV3/GFP、SKOV3细胞迁移至膜下的数目分别为:640±69,316±25,314±37。与SKOV3/GFP、SK-OV3细胞相比,SKOV3/Nlp细胞的迁移能力显著增强(P<0.05)。结论:当Nlp表达增高后,会导致肿瘤细胞的增殖、黏附、侵袭等恶性表型增加,提示Nlp具有促进肿瘤发生发展的生物学作用。
关键词(KeyWords)： Nlp;卵巢肿瘤;中心体异常;肿瘤侵润;肿瘤转移;细胞系,SKOV3
基金项目(Foundation): 973国家重点基础研究发展规划项目基金(No:2002CB513101);; 国家自然科学基金(No:30730046)
作者(Author): 曲丹妮;李媛;张震宇;詹启敏;
Email:
DOI: 10.13283/j.cnki.xdfckjz.2013.02.005
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