子痫前期患者血浆对人脐静脉内皮细胞生长活性及Edg4表达的影响Effects of preeclamptic patients plasma on proliferation and apoptosis of human umbilical vein endothelial cells and the correlation with endothelial differentiation gene-4
李留霞;李秀芳;张兰兰;张毅;
摘要(Abstract)：
目的:探讨子痫前期患者血浆对人脐静脉内皮细胞(HUVECs)生长活性的影响以及与溶血磷脂酸受体蛋白内皮分化基因-4(Edg4)的关系。方法:体外培养HU-VECs,取对数生长的细胞,分组实验:对照组,正常晚孕组,轻度子痫前期组,重度子痫前期组。用MTT比色法和流式细胞仪检测各组细胞增殖及凋亡情况,免疫组化链霉菌抗生物素蛋白-过氧化物酶(SP)法和逆转录聚合酶链反应(RT-PCR)检测各组细胞Edg4蛋白及mRNA表达水平。结果:(1)子痫前期组HUVECs增殖减少,细胞凋亡增加;各组间细胞增殖率及细胞凋亡率差异显著(P<0.05);(2)Edg4蛋白主要表达于HUVECs的细胞浆和细胞膜,子痫前期病情越重,其血浆诱导的HUVECs Edg4蛋白表达越强;(3)子痫前期组Edg4 mRNA表达明显升高,与对照组和正常晚孕组相比显著差异(P<0.05);重度子痫前期组Edg4 mRNA表达较轻度组显著升高(P<0.05)。结论:子痫前期患者血浆可诱导HUVECs Edg4蛋白表达增强,并抑制HUVECs细胞增殖,促进细胞凋亡,提示子痫前期患者溶血磷脂酸升高可能与血管内皮细胞功能异常有关。
关键词(KeyWords)： 子痫前期;血浆;人脐静脉内皮细胞;溶血磷脂素类;受体,溶血磷脂酸;内皮分化基因-4(Edg4);细胞增殖;细胞凋亡
基金项目(Foundation): 河南省教育厅项目(No:2011A320035)
作者(Authors): 李留霞;李秀芳;张兰兰;张毅;
DOI: 10.13283/j.cnki.xdfckjz.2012.10.006
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