Genistein对人卵巢癌细胞系TC-1增殖抑制和诱导凋亡作用的研究Effects of genistein on proliferation inhibition and apoptosis induction in human ovarian carcinoma cell lines TC-1 .
田爽;辛晓燕;黄艳红;胡学昱;袁鹏;张潍;
摘要(Abstract)：
目的:研究植物雌激素染料木黄酮(genistein)对人卵巢癌细胞系TC-1细胞增殖的抑制作用和凋亡的诱导作用,探讨其抗癌作用的机制。方法:应用MTT法检测不同浓度genistein对TC-1细胞的生长抑制作用,电镜观察药物作用后细胞超微结构的改变,流式细胞仪检测细胞周期、定量分析凋亡,DNA琼脂糖凝胶电泳法确定凋亡。结果:TC-1细胞经不同浓度genistein处理后,细胞生长明显受到抑制,且这种抑制作用呈时间及浓度依赖性,5mg/L和10mg/L genistein作用72h后,抑制率分别达到89·84%和97·99%。genistein阻断TC-1细胞生长于G2/M期,在G0/G1前出现典型的亚二倍体凋亡峰,且凋亡呈时间依赖性。电镜观察到用药后凋亡细胞典型的形态学特征。DNA凝胶电泳可观察到细胞凋亡特异的DNA梯形带。结论:genistein对TC-1细胞生长的抑制作用呈明显的时间及浓度依赖性,并诱导其发生凋亡。
关键词(KeyWords)： 染料木黄酮;卵巢肿瘤;人卵巢癌细胞系TC-1;细胞凋亡
基金项目(Foundation): 国家自然科学基金资助项目(30472226)
作者(Authors): 田爽;辛晓燕;黄艳红;胡学昱;袁鹏;张潍;
DOI: 10.13283/j.cnki.xdfckjz.2006.01.006
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