IGFBP-3对子宫内膜癌Ishikawa细胞增殖、凋亡及化疗敏感性的实验研究Effects of insulin-like growth factor binding protein-3(IGFBP-3) on proliferation,apoptosis and chemotherapeutic sensitivity of endometrial carcinoma Ishikawa cell
李艳辉;张晓燕;王泽华;汪宏波;
摘要(Abstract)：
目的:探讨外源性胰岛素样生长因子结合蛋白-3(IGFBP-3)对Ishikawa细胞增殖、凋亡的影响及在抗肿瘤中其能否与顺铂起协同作用,并初步探讨IGFBP-3对垂体肿瘤转化基因1(PTTG1)表达的影响。方法:采用噻唑蓝(MTT)比色法检测IGFBP-3、顺铂对Ishikawa细胞的抑制率,计算半数抑制浓度;用流式细胞仪检测两药单用及合用对Ishikawa细胞周期、凋亡的影响;RT-PCR法检测药物干预后PTTG1表达量的变化。结果:(1)IGFBP-3对Ishikawa细胞增殖有明显抑制作用(IC50=1.00±0.12),且与顺铂有协同作用(CI<1);(2)IGFBP-3干预后Ishikawa细胞凋亡是对照的4.5倍(P<0.05);(3)顺铂、IGFBP-3均能下调PTTG1基因的表达,且以顺铂的下调作用明显。结论:在子宫内膜癌的Ishikawa细胞,观察到IGFBP-3具抗增殖、促凋亡的作用;IGFBP-3可能作为一种抗肿瘤剂用于临床。
关键词(KeyWords)： 胰岛素样生长因子结合蛋白-3;顺铂;Ishikawa细胞;垂体肿瘤转化基因1;细胞凋亡;细胞周期
基金项目(Foundation):
作者(Author): 李艳辉;张晓燕;王泽华;汪宏波;
Email:
DOI: 10.13283/j.cnki.xdfckjz.2008.05.017
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