Efp、VEGF和bFGF在子宫内膜癌的表达及意义Expressions of Efp,VEGF and bFGF in the tissues of endometrial carcinoma.
戴红英;张文华;张晶晶;陈淑明;王波;
摘要(Abstract)：
目的:探讨Efp、VEGF和bFGF在子宫内膜癌组织的表达以及与临床病理参数的关系。方法:采用荧光定量RT-PCR、ELISA和Western blot方法检测Efp、VEGF和bFGF mRNA及蛋白在子宫内膜癌组织的表达。结果:Efp mRNA在子宫内膜癌的表达量为1.12±0.47,低于正常对照组(P(0.05)。VEGF mRNA在子宫内膜癌及不典型增生组的表达量分别为3.20±0.45和2.51±0.37,明显高于正常对照组(P(0.05),bFGFmRNA在子宫内膜癌及不典型增生组的表达量分别为6.43±0.73和3.46±0.62,明显高于正常对照组(P(0.01,P(0.05)。Efp和bFGF mRNA的表达与组织分化程度相关,Efp mRNA在低分化子宫内膜癌的表达低于高分化的表达,而bFGF mRNA的表达则相反。Efp、VEGF和bFGF蛋白的表达与mRNA一致。结论:VEGF和bFGF参与了子宫内膜癌的血管生成,Efp和bFGF在子宫内膜癌的表达变化提示可能与预后有关。
关键词(KeyWords)： 子宫内膜肿瘤;Efp;血管内皮生长因子;碱性成纤维细胞生长因子
基金项目(Foundation):
作者(Authors): 戴红英;张文华;张晶晶;陈淑明;王波;
DOI: 10.13283/j.cnki.xdfckjz.2008.11.014
参考文献(References)：
- [1]Urano T,Saito T,Tsukui T,et al.Efp targets 14-3-3 sigmafor proteolysis and promotes breast tumor growth[J].Na-ture,2002,417:871-875
- [2]Suzuki T,Urano T,Tsukui T,et al.Estrogen-responsivefinger protein as a new potential biomarker for breastcancer[J].Clin Cancer Res,2005,11:6148-6154
- [3]Nakayama H,Sano T,Motegi A,et al.Increasing 14-3-3sigma expression with declining estrogen receptor alphaand estrogen-responsive finger protein expression definesmalignant progression of endometrial carcinoma[J].Pathol Int,2005,55:707-715
- [4]Inoue S,Orimo A,Hosoi T,et al.Genomic binding-sitecloning reveals an estrogen-responsive gene that encodesa RING finger protein[J].Proc Natl Acad Sci U S A,1993,90:11117-11121
- [5]Girling JE,Rogers PA.Recent advances in endometrialangiogenesis research[J].Angiogenesis,2005,8:89-99
- [6]Kamat AA,Merritt WM,Coffey D,et al.Clinical and bio-logical significance of vascular endothelial growth factor inendometrial cancer[J].Clin Cancer Res,2007,13:7487-7495
- [7]Fujimoto J,Hori M,Ichigo S,et al.Expression of basic fi-broblast growth factor and its mRNAin uterine endometri-al cancers[J].Invasion Metastasis,1995,15:203-210
- [8]Hyder SM,Nawaz Z,Chiappetta C,et al.Identification offunctional estrogen response elements in the gene codingfor the potent angiogenic factor vascular endothelialgrowth factor[J].Cancer Res,2000,60:3183-3190
- [9]Goto F,Goto K,Weindel K,et al.Synergistic effects ofvascular endothelial growth factor and basic fibroblastgrowth factor on the proliferation and cord formation ofbovine capillary endothelial cells within collagen gels[J].Lab Invest,1993,69:508-517