高危型HPV16 E6蛋白表达抑制宫颈癌细胞株p63α表达的研究High risk HPV E6 protein inhibits cervical cancer cell lines expression of p63α.
朱靖;范少君;王青元;李敏;李彩荣;肖卫华;周颖;凌斌;
摘要(Abstract)：
目的:探讨高危型HPV16 E6蛋白的表达对宫颈癌细胞株中p63α表达的影响。方法:用RT-PCR法从SiHa中扩增出带酶切位点的HPV16 E6的片段,将HPV16 E6基因片段克隆到p3×flag-CMV-Myc-24真核表达载体上,通过脂质体转染入宫颈癌细胞株后,W estern blot检测p63α的表达。结果:成功构建HPV16 E6真核表达载体,转染至宫颈癌SiHa和Caski细胞株中,p63α均低于对照组。结论:高危型HPV E6能够抑制p63α的表达,提示p63α与HPV E6的相互作用在子宫颈癌发病中发挥重要作用。
关键词(KeyWords)： 宫颈肿瘤;HPV,E6蛋白;p63α;细胞系
基金项目(Foundation): 安徽省自然科学基金项目(No:090413117);; 安徽省高等学校省级自然科学研究项目(No:KJ2010B375)
作者(Authors): 朱靖;范少君;王青元;李敏;李彩荣;肖卫华;周颖;凌斌;
DOI: 10.13283/j.cnki.xdfckjz.2011.06.019
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