张进;席晓薇;张佳荣;蔡斌;杨懿霞;万小平;

• 1:上海交通大学附属第一人民医院妇产科

摘要(Abstract)：

目的:研究与子宫内膜癌淋巴转移相关的蛋白质。方法:提取正常子宫内膜、淋巴结阴性和淋巴结阳性的子宫内膜癌组织总蛋白,用双向凝胶电泳分离蛋白质,用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)分析鉴定差异蛋白点。结果:在淋巴结阴性和淋巴结阳性的子宫内膜癌中,共筛选出11个差异蛋白,与淋巴结阴性组比较,淋巴结阳性内膜癌中,热休克蛋白27、3种肌动蛋白、S100钙结合蛋白A9、丙酮酸激酶、α烯醇化酶、白蛋白和组氨酸三聚体核苷结合蛋白1表达上调,天冬酰胺酶样1蛋白和酯酶D/甲酰-谷胱甘肽水解酶下调。结论:淋巴结阳性和阴性子宫内膜癌的双向凝胶电泳图谱之间存在较明显差异。经鉴定的9种蛋白质可能在子宫内膜癌发生淋巴转移过程中发挥作用。

关键词(KeyWords)： 双向凝胶电泳;基质辅助激光解吸电离飞行时间质谱;子宫内膜癌;淋巴转移

Abstract:

Keywords:

基金项目(Foundation): 上海市卫生局科研基金资助项目(No:044023)

作者(Authors): 张进;席晓薇;张佳荣;蔡斌;杨懿霞;万小平;

DOI: 10.13283/j.cnki.xdfckjz.2009.10.016

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