E6-AP三种转录本在宫颈癌的表达及临床意义E6-AP three kinds of transcripts in cervical carcinoma and its clinical significance
范少君;徐静竹;朱靖;卫莹;凌斌;周颖;
摘要(Abstract)：
目的:探讨E6-AP3种转录本在宫颈癌的表达及其临床意义。方法:免疫组织化学SP法检测30例宫颈癌组织及20例正常宫颈组织中E6-AP蛋白的表达,分析E6-AP的表达与宫颈癌病理分级之间的关系。同时用W estern blot检测E6-AP在正常宫颈脱落细胞和宫颈癌脱落细胞的表达水平。RT-PCR方法明确E6-AP 3种转录本在正常宫颈组织和宫颈癌组织中的表达。结果:RT-PCR结果显示E6-AP3种亚型在宫颈癌组织中均存在,并且表达高于正常子宫颈组织,其中Ⅱ型表达水平很低,以Ⅰ型和Ⅲ型为主。免疫组化和W estern B lot结果显示E6-AP在宫颈癌组织表达水平高于正常宫颈组织。结论:E6-AP表达升高可能与宫颈癌的发病机制相关。
关键词(KeyWords)： 宫颈肿瘤;E6相关蛋白;泛素
基金项目(Foundation): 安徽省自然科学基金资助项目(No:090413117);; 安徽省高等学校省级自然科学研究资助项目(No:KJ2010B375)
作者(Authors): 范少君;徐静竹;朱靖;卫莹;凌斌;周颖;
DOI: 10.13283/j.cnki.xdfckjz.2010.10.012
参考文献(References)：
- [1]Talis AL,Huibregtse JM,Howley PM.The role of E6AP inthe regulation of p53 protein levels in human papilloma-virus(HPV)-positive and HPV-negative cells[J].J BiolChem,1998,273:6439-6445
- [2]Yamamoto Y,Huibregtse JM,Howley PM.The human E6-AP gene(UBE3A)encodes three potential protein isoformsgenerated by differential splicing[J].Genomics,1997,41:263-266
- [3]Mishra A,Godavarthi SK,Maheshwari M,etal.The ubiq-uitin ligase E6-AP is induced and recrui ted to aggre-somes in response to proteasome inhibition and may be in-volved in the ubiquitination of Hsp70-bound misfoldedproteins[J].J Biol Chem,2009,284:10537-10545
- [4]朱靖,周颖,凌斌.野生型p53及其调控基因与子宫颈癌发病机制[J].国外医学妇产科分册,2010,3:187-189
- [5]Liu X,Yuan H,Fu B,et al.The E6-AP ubiquitin ligase isrequired for transactivation of the hTERTpromoter by thehuman papillomavirus E6 oncoprotein[J].J Biol Chem,2005,280:10807-10816
- [6]James MA,Lee JH,Klingelhutz AJ.HPV16-E6 associatedhTERT promoter acetylation is E6-AP dependent,in-creased in later passage cells and enhanced by loss ofp300[J].Int J Cancer,2006,119:1878-1885
- [7]Williams C.Angelman syndrome scientific symposium onthe structure and function of UBE3A/E6-AP[J].J ChildNeurol,2009,24:904-908
- [8]周颖,卫莹,凌斌.E6-AP与蛋白降解[J].医学分子生物学杂志,2009,6:447-450