主改霞;王冬颖;吴静;

• 1:西安交通大学医学院第二附属医院妇产科
• 2:西安交通大学医学院第二附属医院妇产科
• 3:西安交通大学医学院第二附属医院妇产科 西安710004
• 4:西安710004

摘要(Abstract)：

目的:应用小鼠月经模型,通过免疫组织化学及计算机辅助图像分析系统(CAST2)研究巨噬细胞和T淋巴细胞随月经周期在子宫内膜的分布变化以及在子宫内膜所占体积分数(volume fraction)的变化。方法:用成年雌性去势C57BL/6小鼠34只,给予序贯性激素处理,在最末次激素处理后4⁶h,实验组小鼠宫腔内注射0.2ml消毒花生油诱导子宫内膜蜕膜化反应,对照组小鼠给予同样序贯性激素处理但无宫腔油剂注射。分别取出各组子宫内膜组织,进行常规HE染色,以F4/80作为巨噬细胞的标记分子,CD4和CD8作为T淋巴细胞的标记分子进行免疫组织化学染色,用计算机辅助图像分析软件CAST2计算分析巨噬细胞、CD4和CD8T淋巴细胞和在子宫内膜组织中所占的体积分数。结果:HE染色子宫内膜组织切片显示激素撤退后实验组在宫腔内注射油剂后306h,实验组小鼠宫腔内注射0.2ml消毒花生油诱导子宫内膜蜕膜化反应,对照组小鼠给予同样序贯性激素处理但无宫腔油剂注射。分别取出各组子宫内膜组织,进行常规HE染色,以F4/80作为巨噬细胞的标记分子,CD4和CD8作为T淋巴细胞的标记分子进行免疫组织化学染色,用计算机辅助图像分析软件CAST2计算分析巨噬细胞、CD4和CD8T淋巴细胞和在子宫内膜组织中所占的体积分数。结果:HE染色子宫内膜组织切片显示激素撤退后实验组在宫腔内注射油剂后30³5h可观察到子宫内膜脱落,免疫组织化学组织切片显示在激素撤退早期实验组与对照组子宫内膜内的巨噬细胞均增加并且分布无明显差别(P=0.642),但在激素撤退晚期实验组子宫内膜内的巨噬细胞无明显减少(P=0.061),对照组与之相比则明显减少(P=0·032)。CD8T淋巴细胞的增多仅出现于激素撤退早期实验组子宫内膜。CD4T淋巴细胞在子宫内膜的数量很少且不随月经周期变化。结论:蜕膜化的子宫内膜在激素撤退后将导致内膜的脱落与白细胞的侵入,且数量与分布的变化与激素撤退的时间明显相关。

关键词(KeyWords)： 月经模型,小鼠;巨噬细胞;T淋巴细胞;体积分数

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 主改霞;王冬颖;吴静;

DOI: 10.13283/j.cnki.xdfckjz.2006.09.008

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