LHRH拮抗剂Cetrorelix对子宫内膜癌细胞生长周期的影响及其机理The effect of LHRH antagonist cetrorelix on the cell cycle of endometrial carcinoma and its mechanism
梁睿,汤晓晖,主改霞,张建国
摘要(Abstract)：
目的 :研究LHRH拮抗剂Cetrorelix对子宫内膜癌细胞生长周期及周期相关蛋白的影响 ,探讨其抑制内膜癌细胞生长的机理。方法 :用流式细胞仪细胞周期分析及Westernblotting蛋白分析法 ,研究在Cetrorelix的作用下子宫内膜癌细胞系HEC 1A细胞生长周期及相关周期蛋白的改变。结果 :1 0 -5mol/LCetrorelix可导致HEC 1A细胞生长停滞于G2 /M期 ,而与G2 /M期停滞相关的p5 3 ,磷酸化p5 3 (phospho p5 3 ) (丝氨酸 3 92 )及磷酸化cdc2 (phospho cdc2 ) (酪氨酸 1 5 )蛋白水平均显著增高。结论 :Cetrorelix抑制内膜癌细胞增殖作用的机理是结合细胞表面受体后引起一系列抑制性信号传递 ,导致细胞周期停滞于G2 /M期 ,主要表现为G2 期停滞。其中p5 3激活及cdc2磷酸化失活是引起细胞周期停滞的重要因素
关键词(KeyWords)： 子宫内膜肿瘤;促性腺素释放激素;细胞周期;蛋白质P53;cdc2蛋白激酶
基金项目(Foundation):
作者(Author): 梁睿,汤晓晖,主改霞,张建国
DOI: 10.13283/j.cnki.xdfckjz.2003.05.010
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