下调microRNA-205表达对人子宫内膜癌Ishikawa细胞增殖和侵袭的作用Effects of abrogating expression of miR-205 on proliferation and migration of endometrial carcinoma Ishikawa cells and its mechanism
苏宁;陈一飞;严沁;万小平;
摘要(Abstract)：
目的:探讨下调microRNA-205(miR-205)表达对人子宫内膜样腺癌细胞系Ishika-wa增殖和侵袭的作用及可能的机制。方法:将miR-205抑制剂(miR-205 inhibitor)瞬时转染Ishikawa细胞,下调miR-205的表达;应用实时荧光定量PCR方法(qRT-PCR)检测miR-205的表达;噻唑蓝(MTT)法检测细胞增殖能力;Transwell小室法检测细胞侵袭能力。qRT-PCR和Western blot分别检测miR-205的预测靶基因ESRRG mRNA和蛋白表达水平。应用双荧光素酶分析方法鉴定miR-205和ESRRG 3'UTR的表达调节关系。结果:miR-205在Ishikawa细胞中呈高表达;转染miR-205 inhibitor的Ishikawa细胞中,miR-205表达降低了86.9%,细胞增殖和侵袭能力下降,同时细胞ESRRG mRNA和蛋白表达升高;miR-205通过直接与ESRRG mR-NA 3'UTR结合负调节其表达。结论:下调miR-205表达可抑制Ishikawa细胞增殖、侵袭能力;miR-205的生物效应可能与调节潜在抑癌基因ESRRG有关。
关键词(KeyWords)： 子宫内膜肿瘤;微小RNA;子宫内膜样腺癌;ESRRG;miR-205;增殖;侵袭
基金项目(Foundation): 国家自然科学基金资助项目(No:81072139)
作者(Authors): 苏宁;陈一飞;严沁;万小平;
DOI: 10.13283/j.cnki.xdfckjz.2012.07.020
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