亚硝酸钠对地鼠生殖毒性的研究The study about the reproductive toxicity reaction of sodium nitrite on the hamster.
顾宪敏;马保华;禹卉;董平;
摘要(Abstract):
目的:探讨亚硝酸钠的生殖毒性。方法:(1)体内实验:随机将未经产雌性成熟金黄地鼠160只分为对照组和实验组,实验组分别腹腔注射不同浓度的亚硝酸钠[2、4、8mg/(kg·d)],对照组给予生理盐水。于光镜及电镜下观察卵巢颗粒细胞的形态改变;(2)体外实验:细胞培养并对培养的卵巢颗粒细胞进行钙通道蛋白免疫印迹半定量实验以观察钙通道蛋白的表达量。结果:(1)予以亚硝酸钠4mg/(kg·d)和8mg/(kg·d)负荷5天后,地鼠卵巢有毛细血管扩张充血、卵巢颗粒细胞排列紊乱等明显变化;(2)予以亚硝酸钠8mg/(kg·d)负荷5天后,地鼠有卵泡透明带变窄、其内(颗粒细胞)微绒毛减少、颗粒细胞内脂滴和滑面内质网也减少等明显变化;(3)体外培养系统内追加不同负荷剂量的亚硝酸钠24h后,培养的颗粒细胞相应的钙通道蛋白表达呈剂量依赖性下降(P<0.05 vs对照组);(4)腹腔内注射不同负荷剂量的亚硝酸钠5天后,相应的地鼠合笼交配后生仔率也呈现剂量依赖性下降(P<0.05 vs对照组)。结论:亚硝酸钠影响地鼠卵巢颗粒细胞正常结构的发育,干扰地鼠生育能力。
关键词(KeyWords): 亚硝酸钠;钙通道蛋白;卵巢颗粒细胞;生育能力;地鼠
基金项目(Foundation): 山东省自然科学基金资助课题(No:Y2004C08)
作者(Authors): 顾宪敏;马保华;禹卉;董平;
DOI: 10.13283/j.cnki.xdfckjz.2008.04.006
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