miR-31与宫颈癌淋巴转移的相关性研究Study of miR-31 in cerivical cancer with lymph node metastasis
黄晨;赵群;吴玉梅;
摘要(Abstract)：
目的:研究微RNAs(miRNAs)差异表达与宫颈癌淋巴转移的关系,寻找早期预测淋巴转移的分子标志物及判断患者预后的指标,探讨宫颈癌淋巴转移可能的作用机制。方法:应用安捷伦(Agilent)Human miRNAs V16.0基因芯片检测宫颈癌淋巴转移组与非淋巴转移组的差异表达miRNAs基因,筛选宫颈癌淋巴转移相关miRNAs,并采用实时荧光PCR技术(Real-time PCR)验证芯片结果的准确性,用生物信息学法分析其作用靶点。结果:宫颈癌淋巴转移组中,共筛选出3个显著下调的miRNAs(P<0.05):hsa-miR-31、hsa-miR-31*、hsa-let-7d。其中,hsa-miR-31是宫颈癌淋巴转移组下调最显著的miRNAs。实时荧光PCR验证结果与芯片结果基本吻合。结论:hsa-miR-31抑制肿瘤细胞生长、迁移,在淋巴转移宫颈癌组织中的表达明显下调,它可能成为宫颈癌淋巴转移的分子标志物及治疗靶点。
关键词(KeyWords)： 子宫颈肿瘤;癌,鳞状细胞;基因,肿瘤抑制;微RNAs;miR-31;淋巴转移;基因芯片
基金项目(Foundation): 首都医科大学基础与临床科研合作基金(No:10JL60)
作者(Author): 黄晨;赵群;吴玉梅;
Email:
DOI: 10.13283/j.cnki.xdfckjz.2013.02.013
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