5-杂氮脱氧胞苷对JEG-3细胞及印迹基因H19效应的初步研究The expression of imprinted gene H19 and the demethylation effect of 5-aza-2'-deoxycytidine in a choriocarcinoma cell line
卢林杉;李力;俞丽丽;易萍;李平;陈星云;刘苹;周元国;
摘要(Abstract)：
目的:研究甲基化酶抑制剂5-杂氮脱氧胞苷(5-aza-2'-deoxycytidine,ADC)对人绒毛膜上皮癌细胞株JEG-3细胞的生物学作用,观察JEG-3细胞H19表达变化与滋养细胞增殖、分化和侵袭等行为的相关性,从表观遗传学角度探讨滋养细胞侵袭行为的调控。方法:应用MTT法检测不同浓度5-杂氮脱氧胞苷在不同时相下对JEG-3细胞增殖的影响。体外小室迁移实验观察5-杂氮脱氧胞苷对JEG-3细胞迁移的影响。荧光定量RT-PCR检测5-杂氮脱氧胞苷作用下JEG-3细胞H19mRNA表达的变化。结果:5-杂氮脱氧胞苷对JEG-3细胞增殖起抑制作用,在一定范围内与时间和剂量呈正相关。经100μmol/L5-杂氮脱氧胞苷作用24h后的JEG-3细胞迁移能力明显受限,迁移细胞数较对照组差异显著(P<0.01)。经5-杂氮脱氧胞苷作用后JEG-3细胞中H19mRNA表达增高,在一定范围内与时间和剂量呈正相关。结论:甲基化酶抑制剂5-杂氮脱氧胞苷可致H19mRNA过量表达,并进一步抑制滋养细胞的增殖和迁移能力。H19印迹基因表达上调与滋养细胞增殖抑制和迁移能力降低有关。
关键词(KeyWords)： 绒毛膜癌;脱氧胞苷;印迹基因H19;细胞JEG-3;迁移,细胞系
基金项目(Foundation): 国家自然科学基金资助项目(No:30500542;30772338)
作者(Authors): 卢林杉;李力;俞丽丽;易萍;李平;陈星云;刘苹;周元国;
DOI: 10.13283/j.cnki.xdfckjz.2008.05.027
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