李望舒;白治苗;王瑞;卢玉凤;陈华;姚卫卫;哈春芳;

• 1:宁夏医科大学
• 2:宁夏医科大学总医院妇产科
• 3:宁夏医科大学生育力保持教育部重点实验室

摘要(Abstract)：

目的:探讨RNAi抑制核因子NF-κB p65对子宫内膜异位症(EMs)在位内膜腺上皮细胞中OPN、MMP-9表达及细胞侵袭性的影响。方法:采用NF-κB p65 siRNA基因沉默转染10例EMs患者在位内膜原代腺上皮细胞,由Western blot、Real-time PCR、Transwell等分别检测干扰前后OPN、NF-κB p65、MMP-9蛋白和mRNA表达及细胞侵袭性的变化。结果:与未干预组比较,NF-κB p65 siRNA干预后EMs腺上皮细胞中OPN蛋白及mRNA表达无明显变化,差异无统计学意义(P均>0.05);NF-κB p65、MMP-9蛋白及mRNA表达降低,差异均有统计学意义(P均<0.05)。NF-κB p65 siRNA干预后,EMs腺上皮细胞的细胞侵袭性明显降低,差异有统计学意义(P<0.05)。结论:揭示NF-κB可能通过下调MMP-9的表达进而改变细胞侵袭性诱导EMs的发生。

关键词(KeyWords)： 子宫内膜异位症;核因子NF-κB p65;骨桥蛋白;金属基质蛋白酶-9;细胞侵袭性45

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No:81160078);; 2015年宁夏研究生教育创新计划项目(No:NXYC201511)

作者(Authors): 李望舒;白治苗;王瑞;卢玉凤;陈华;姚卫卫;哈春芳;

DOI: 10.13283/j.cnki.xdfckjz.2016.07.034

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