拓扑替康对宫颈癌细胞系HeLa放射增敏作用的研究Radiosensitization effect of Topotecan on cervical carcinoma cell line HeLa.
唐丽萍;徐向英;娄阁;李晓梅;
摘要(Abstract)：
目的:探讨拓扑替康对宫颈癌细胞系HeLa的放射增敏作用及其作用机制。方法:用MTT法和克隆形成分析实验检测拓扑替康对HeLa细胞放射增敏的影响,流式细胞仪检测细胞凋亡率及细胞周期。结果:放疗同时给予0.1、0.5、1.0、5.0μg/m l拓扑替康的放射增敏比分别为1.21、1.40、1.66、1.96;放疗同时及放疗后3h给予拓扑替康组增敏效果最佳,放疗前和放疗后6h以后给予拓扑替康组细胞生存分数与此差异有统计学意义(P<0.05);流式细胞仪检测单化组、单放组和增敏组放疗后24h和48h细胞凋亡率分别为:12.30%和17.00%,16.13%和21.90%,25.13%和34.43%;单放组和不同剂量拓扑替康增敏组G2/M期细胞比例增加。结论:拓扑替康对宫颈癌细胞系HeLa有放射增敏作用,且有剂量依赖性和用药时间选择性,其放射增敏机制可能与HeLa细胞放射后损伤性修复、细胞凋亡及G2/M期阻滞相关。
关键词(KeyWords)： 宫颈肿瘤;HeLa细胞;拓扑替康;辐射增敏药;细胞凋亡
基金项目(Foundation):
作者(Authors): 唐丽萍;徐向英;娄阁;李晓梅;
DOI: 10.13283/j.cnki.xdfckjz.2006.05.004
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