梁升;陆晓媛;

• 1:徐州医科大学研究生学院妇产科

摘要(Abstract)：

目的:探究腺病毒介导的Fat-1基因(Ad-GFP-Fat-1)对人子宫内膜癌Ishikawa细胞体内抑制效应及其潜在的分子机制。方法:体外培养人子宫内膜癌Ishikawa细胞,接种于裸鼠右前肢腋部,建立人子宫内膜癌裸鼠皮下移植瘤模型。将成瘤后裸鼠随机分成3组:Ad-GFP-Fat-1实验组、Ad-GFP空载体组、PBS对照组,分别对裸鼠进行皮下移植瘤注射治疗。观察并测量瘤体体积。RT-PCR检测Fat-1基因表达,TUNEL+DAPI染色法检测移植瘤组织凋亡,免疫组化SP法和Western blot检测瘤体内半胱氨酸蛋白酶(Caspase)-3的表达情况。结果:Fat-1基因在Ad-GFP-Fat-1组移植瘤组织中有效异源表达。Ad-GFP-Fat-1组裸鼠移植瘤的体积和重量明显小于Ad-GFP组和PBS组(瘤体比较:P<0.05,瘤重比较:P<0.05)。Ad-GFP-Fat-1组的凋亡指数明显高于Ad-GFP组和PBS组(P<0.05)。Ad-GFP-Fat-1可明显上调Caspase-3表达。Ad-GFP-Fat-1主要通过增加活化的Caspase-3(Cleaved Caspase-3)上调Caspase-3表达。结论:Ad-GFP-Fat-1表达对人子宫内膜癌裸鼠皮下移植瘤有抑制作用,其机制可能与Fat-1基因上调Cleaved Caspase-3表达诱导细胞凋亡有关。

关键词(KeyWords)： 腺病毒载体;裸鼠;Fat-1基因;Caspase-3;子宫内膜癌

Abstract:

Keywords:

基金项目(Foundation): 江苏省卫生厅医学科研项目(No:H201428)

作者(Authors): 梁升;陆晓媛;

DOI: 10.13283/j.cnki.xdfckjz.2017.05.004

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