李欣然;何援利;王雪峰;彭冬先;陈小莹;王清;付霞霏;

• 1:南方医科大学珠江医院妇产科

摘要(Abstract)：

目的:探讨过表达miR-21的BMMSCs对化疗性卵巢早衰的治疗作用及可能机制。方法:采用分子生物学方法构建miR-21慢病毒表达载体(LV-miR-21)。使用LV-miR-21转染体外分离培养的大鼠BMMSCs,获得过表达miR-21的BMMSCs(miR-21-BMMSCs)。建立化疗性卵巢早衰大鼠模型,双侧卵巢注射miR-21-BMMSCs,分别于注射后第15天、30天、45天、60天分批处死大鼠。采用阴道涂片测定动情周期,卵巢称重,HE染色进行卵泡计数,化学发光法测定性激素水平,TUNEL法测定卵巢颗粒细胞凋亡率,qRT-PCR、Western blot法检测miR-21及PTEN、PDCD4的mRNA及蛋白水平。结果:双侧卵巢注射miR-21-BMMSCs后,化疗诱导的POF大鼠动情周期恢复率、卵巢重量、各级卵泡数和E2水平增加,而FSH水平及卵巢颗粒细胞凋亡率下降。此外,miR-21表达水平的上升伴随PTEN、PDCD4 mRNA及蛋白水平的下降。结论:过表达miR-21的BMMSCs能更有效地治疗化疗性卵巢早衰,可能与其调控靶基因PTEN、PDCD4密切相关。

关键词(KeyWords)： microRNA-21;骨髓间充质干细胞;卵巢颗粒细胞;卵巢早衰;化疗

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No:81300462)

作者(Authors): 李欣然;何援利;王雪峰;彭冬先;陈小莹;王清;付霞霏;

DOI: 10.13283/j.cnki.xdfckjz.2018.12.010

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