卵巢肿瘤p16、Rb、CyclinD1基因蛋白的流式细胞分析及其与细胞增殖的关系Flow cytometric measurement of p16, Rb, CyclinD1 protein in ovarian neoplasms and their correlation with cell proliferation
李莉,苏杏满,程建新,郭建文,刘江惠,杨静
摘要(Abstract)：
目的：探讨卵巢肿瘤中p16、Rb、CyclinD1基因蛋白的表达及其与细胞增殖的关系。方法：利用流式细胞术检测60份卵巢肿瘤组织中p16、Rb、CyclinD1基因蛋白的表达，同时检测DNA的含量，以5份正常卵巢组织作对照。结果：从良性到恶性，p16、Rb的荧光指数（FI）依次降低，CyclinD1表达依次升高，增殖指数（PI）亦升高。阳性组P16的PI低于阴性组，多元线性回归显示p16与PI呈负相关（P＝0．0081）；Rb、CyclinD1不同表达时，PI值有差异，但无统计学意义。p16、Rb阳性表达时，DNA指数（DI）下降，而CyclinD1阳性表达时，DI值较高。结论：p16、Rb缺失，CyclinD1增加可促进肿瘤的恶性转化及异常增殖。
关键词(KeyWords)： 卵巢肿瘤;基因.p16;基因.Rb;基因.CyclinD1;细胞增殖;流式细胞术
基金项目(Foundation):
作者(Author): 李莉,苏杏满,程建新,郭建文,刘江惠,杨静
DOI: 10.13283/j.cnki.xdfckjz.1999.04.012
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