人重组IFN-γ对STAT-1在卵巢癌细胞中表达调控的研究Effect of recombinant human IFN-γ on the expression of STAT-1 in ovarian cancer cells
沈怡;方大俊;阮莎莎;赵虹;王泽华;
摘要(Abstract)：
目的:研究IFN-γ对卵巢癌耐药细胞株SKOV3TS及敏感细胞株SKOV3中STAT-1表达的调控。方法:应用RT-PCR方法、细胞免疫荧光法检测SKOV3TS与SKOV3在IFN-γ作用前后STAT-1 mRNA及蛋白的表达。用MTT法检测不同浓度IFN-γ作用卵巢癌细胞株不同时间后对细胞增殖率的影响。结果:卵巢癌SKOV3TS、SKOV3细胞株均有STAT-1表达,且主要集中于胞浆。并且随着IFN-γ作用时间延长,STAT-1蛋白表达增强。IFN-γ作用两种细胞株24h及48h后STAT-1 mRNA表达均增加,且差异有统计学意义(P<0.01),但不同浓度IFN-γ作用相同时间后STAT-1 mRNA表达差异无统计学意义(P>0.05)。IFN-γ可显著抑制SKOV3TS和SKOV3细胞增殖,也呈时间依赖性(P<0.01)。IFN-γ对SKOV3组的抑制率较SKOV3TS组高(P<0.05)。结论:IFN-γ能够促进STAT-1表达,抑制卵巢癌细胞增殖。
关键词(KeyWords)： 卵巢肿瘤;细胞系;人重组IFN-γ;STAT-1;噻唑蓝;聚合酶链反应,逆转录酶
基金项目(Foundation): 湖北省科技攻关项目基金资助(No:4-367)
作者(Authors): 沈怡;方大俊;阮莎莎;赵虹;王泽华;
DOI: 10.13283/j.cnki.xdfckjz.2010.02.023
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