GnRHⅡ对离体培养子宫内膜异位症间质细胞凋亡的影响Effect of GnRHⅡon apoptosis of the stromal cells from endometriosis in vitro
黄凤英;邹颖;王焕萍;刘秋红;方小玲;林秋华;
摘要(Abstract)：
目的:研究GnRHⅡ对子宫内膜异位症(内异症)患者离体培养的异位及在位子宫内膜间质细胞凋亡的影响。方法:体外原代培养人内异症异位及在位子宫内膜间质细胞,用不同浓度的GnRHⅡ(0、10-10、10-8、10-6mol/L)干预,采用Hoechst染色和流式细胞术检测离体培养子宫内膜间质细胞的凋亡发生情况。结果:GnRHⅡ可诱导离体培养的在位或异位子宫内膜间质细胞的凋亡,随着浓度增加,差异有统计学意义(P<0.05),呈剂量依赖性;且对异位内膜间质细胞的促凋亡作用明显强于在位者(P<0.05)。结论:外源性GnRHⅡ可明显促进人内异症异位及在位子宫内膜间质细胞的凋亡,为开发内异症促凋亡治疗方面的新药提供了实验依据。
关键词(KeyWords)： 子宫内膜异位症;促性腺素释放激素Ⅱ;细胞凋亡;间质细胞;细胞培养
基金项目(Foundation):
作者(Authors): 黄凤英;邹颖;王焕萍;刘秋红;方小玲;林秋华;
DOI: 10.13283/j.cnki.xdfckjz.2010.06.001
参考文献(References)：
- [1]Tesone M,Bilotas M,Baranao RI,et al.The role of GnRHanalogues in endometriosis-associated apoptosis and angio-genesis[J].Gynecol Obstet Invest,2008,66:8-10
- [2]Grundker C,Gunthert AR,Millar RP,et al.Expression ofgonadotropin-releasing hormoneⅡ(GnRH-Ⅱ)receptorin human endometrial and ovarian cancer cells and effectsof GnRH-Ⅱon tumor cell proliferation[J].J Clin Endo-crinol Metab,2002,87:1427-1430
- [3]Morimoto C,Osuga Y,Yano T,et al.GnRHⅡas a possi-ble cytostatic regulator in the development of endometriosis[J].Hum Reprod,2005,20:3212-3218
- [4]苑春丽,韩丽英,胡静,等.人子宫内膜间质细胞的体外培养[J].吉林大学学报,2004,3:267-269
- [5]乐杰.妇产科学[M].北京:人民卫生出版社,2008,326
- [6]黄凤英,方小玲,林秋华.Bcl-2和bax蛋白在正常子宫内膜中的表达[J].中华妇产科杂志,2000,35:183
- [7]韩利伟,姜卫国.GnRHa对子宫内膜异位症内膜间质细胞生长增殖及血管形成的影响[J].现代妇产科进展,2008,17:737-739
- [8]Devrim A,Arif K,Migraci T,et al.Comparison of theeffects of cetrorelix,a GnRH antagonist,and leuprolide,aGnRH agonist,on experimental endometriosis[J].J Ob-stet Gynaecol Res,2008,34:1014-1019
- [9]Bilotas M,Bara ao RI,Buquet R,et,al.Effect of GnRHanalogues on apoptosis and expression of Bcl-2,Bax,Fasand FasL proteins in endometrial epithelial cell culturesfrom patients with endometriosis and controls[J].HumReprod,2007,22:644-653
- [10]Millar RP.GnRHⅡand typeⅡGnRH receptors[J].Trends Endocrinol Metab,2003,14:35-43
- [11]Harrison GS,Wierman ME,Nett TM,et al.Gonadotropin-releasing hormone and its receptor in normal and malig-nant cells[J].Endocr Relat Cancer,2004,11:725-748
- [12]Fister S,Gunthert AR,Emons G,et al.Gonadotropin-re-leasing hormone typeⅡantagonists induce apoptotic celldeath in human endometrial and ovarian cancer cellsinvitroand in vivo[J].Cancer Res,2007,67:1750-1756