抗子宫内膜抗体对人早孕绒毛滋养细胞凋亡及FasL蛋白表达的影响Effects of anti-endometrium antibody on AI and FasL expression of cultured human cytotrophoblast cells
王永萍;徐永萍;邱健青;马小静;
摘要(Abstract)：
目的:探讨体外培养人早孕绒毛滋养层细胞在游离抗子宫内膜抗体(Em-Ab)环境中,细胞凋亡指数(apoptosis index,AI)及凋亡相关因子配体(factor associated suicide ligend,FasL)的表达。方法:培养经胰蛋白酶/DNA酶Ⅰ联合消化,通过Percoll细胞分离液纯化得到绒毛滋养细胞。用TUNEL法测定EmAb(+)血清处理组和EmAb(-)血清处理组绒毛滋养细胞培养0、48、72、96h时的细胞凋亡指数,同时用免疫细胞化学法测定FasL蛋白的表达。结果:EmAb(+)血清处理组于培养72、96h时间点测得的AI明显高于EmAb(-)血清处理组(P<0.01);FasL蛋白表达明显低于EmAb(-)血清处理组;而培养48h AI及FasL蛋白表达无显著差异。结论:绒毛滋养细胞在EmAb(+)环境下AI增加,FasL蛋白表达减少。
关键词(KeyWords)： 绒毛滋养细胞;细胞凋亡指数;凋亡相关因子配体
基金项目(Foundation): 山东省计生委科研基金资助项目
作者(Author): 王永萍;徐永萍;邱健青;马小静;
Email:
DOI: 10.13283/j.cnki.xdfckjz.2008.12.014
参考文献(References)：
- [1]席娜,许良智.细胞凋亡与流产[J].四川医学,2005,26:803-805
- [2]童英,赵磐琳,任力,等.自然流产蜕膜、绒毛组织细胞凋亡与p53、Bax、Bcl-2蛋白表达的关系[J].实用医学杂志,2008,24:189-190
- [3]刘玉昆,张建平,邝健全,等.复发性自然流产患者绒毛滋养细胞凋亡相关蛋白配体的表达[J].中华妇产科杂志,2005,40:126-127
- [4]Kliman HJ,Nestler JE,Sermasi E,et al.Purification,char-acterization,and in vitro differentiation of cytotrophoblasts from human term placentae[J].Endocrinology,1986,118:1567-1582
- [5]Daya S,Gunby J,Clark DA.Intravenous immunoglobulin therapy for recurrent spontaneous abortion:a meta-analysis[J].Am J Repord Immunol,1998,39:69-76
- [6]贾莉婷,李培育,任芬若,等.原因不明习惯性流产患者抗子宫内膜抗体和肿瘤坏死因子α的测定[J].中国免疫学杂志,1999,15:45-46
- [7]Runic R,Lockwood CJ,Ma Y,et al.Expression of Fas lig-and by human cytotrophoblasts:implications in placenta-tion and fetal survival[J].J Clin Endocrinol Metab,1996,81:3119-3122
- [8]Uckan D,Steele A,Wang BY,et al.Trophoblasts express Fas ligand:a proposed mechanism for immune privilege in placenta and maternal invasion[J].Mol Hum Reprod,1997,3:655-662
- [9]Kauma SW,Huff TF,Hayes N,et al.Placental Fas ligand expression is a mechanism for maternal immune tolerance to the fetus[J].J Clin Endocrinol Metab,1999,84:2188-2194