抗子宫内膜抗体对人早孕绒毛滋养细胞凋亡及FasL蛋白表达的影响Effects of anti-endometrium antibody on AI and FasL expression of cultured human cytotrophoblast cells
王永萍;徐永萍;邱健青;马小静;
摘要(Abstract)：
目的:探讨体外培养人早孕绒毛滋养层细胞在游离抗子宫内膜抗体(Em-Ab)环境中,细胞凋亡指数(apoptosis index,AI)及凋亡相关因子配体(factor associated suicide ligend,FasL)的表达。方法:培养经胰蛋白酶/DNA酶Ⅰ联合消化,通过Percoll细胞分离液纯化得到绒毛滋养细胞。用TUNEL法测定EmAb(+)血清处理组和EmAb(-)血清处理组绒毛滋养细胞培养0、48、72、96h时的细胞凋亡指数,同时用免疫细胞化学法测定FasL蛋白的表达。结果:EmAb(+)血清处理组于培养72、96h时间点测得的AI明显高于EmAb(-)血清处理组(P<0.01);FasL蛋白表达明显低于EmAb(-)血清处理组;而培养48h AI及FasL蛋白表达无显著差异。结论:绒毛滋养细胞在EmAb(+)环境下AI增加,FasL蛋白表达减少。
关键词(KeyWords)： 绒毛滋养细胞;细胞凋亡指数;凋亡相关因子配体
基金项目(Foundation): 山东省计生委科研基金资助项目
作者(Authors): 王永萍;徐永萍;邱健青;马小静;
DOI: 10.13283/j.cnki.xdfckjz.2008.12.014
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