赖志文;罗爱月;杨书红;丁婷;卢运萍;马丁;王世宣;

• 1:华中科技大学同济医学院附属同济医院妇产科

摘要(Abstract)：

目的:探讨Egr-1、Gadd45α在小鼠卵泡发育不同阶段的表达及其相互关系。方法:选择不同发育阶段的昆明种小鼠卵巢,用免疫组化法检测不同发育阶段卵泡中Egr-1、Gadd45α蛋白的表达。结果:Egr-1、Gadd45α蛋白在卵泡不同发育阶段的卵母细胞及颗粒细胞中均有表达,而且随卵泡发育表达增强。卵泡不同发育阶段中卵母细胞及颗粒细胞中Egr-1、Gadd45α蛋白表达差异有统计学意义(P<0.05)。闭锁卵泡Egr-1、Gadd45α蛋白表达强烈,其表达强度明显高于各级生长卵泡。黄体及间质中亦有Egr-1、Gadd45α表达。同时Egr-1、Gadd45α在各级卵母细胞的表达有较强的相关性(rs=0.993,P<0.05)。结论:卵泡发育不同阶段Egr-1、Gadd45α均有表达,在卵泡发育后期和闭锁卵泡中强表达。提示Egr-1、Gadd45α在卵泡生长发育及闭锁过程中可能起重要的调控作用。

关键词(KeyWords)： 早期生长反应基因;生长阻滞和DNA损伤诱导基因;卵泡发育;免疫组织化学;小鼠

Abstract:

Keywords:

基金项目(Foundation): 卫生部卫生公益性行业科研专项经费项目资助(No:200802159);; 教育部高等学校博士点科研基金(No:200804870009)

作者(Authors): 赖志文;罗爱月;杨书红;丁婷;卢运萍;马丁;王世宣;

DOI: 10.13283/j.cnki.xdfckjz.2009.11.005

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