张军;蒋惠萍;陈咏宁;王丽婧;刘春花;郑曰燕;龚时鹏;郭遂群;

• 1:南方医科大学南方医院妇产科
• 2:南方医科大学第三附属医院

摘要(Abstract)：

目的:探讨fat-1基因对宫颈癌Hela细胞生长的影响,并研究PI3K/Akt/mTOR通路是否参与fat-1对Hela细胞生长的调控作用。方法:构建真核表达载体(p-EGFP-fat-1),并转染人宫颈癌Hela细胞,使其过表达fat-1基因,为fat-1组,以转染空载质粒的Hela细胞为阴性对照组,以未经任何处理的Hela细胞为空白对照组。采用气相色谱法检测fat-1基因对ω-3多不饱和脂肪酸(ω-3PUFA)和ω-6多不饱和脂肪酸(ω-6PUFA)含量的影响;采用MTT和Hoechst实验检测fat-1基因对细胞增殖和凋亡的影响;采用Transwell和细胞划痕实验检测fat-1基因对细胞侵袭和迁移的影响;采用Western blot检测fat-1基因对PI3K/Akt/mTOR通路相关蛋白表达情况的影响。结果:Fat-1组细胞中fat-1基因表达水平高于空白对照组和阴性对照组(P<0.01)。Fat-1组细胞中ω-3PUFA含量高于空白对照组和阴性对照组,ω-6PUFA含量和ω-6PUFA/ω-3PUFA比例均低于空白对照组和阴性对照组(P均<0.01)。Fat-1组细胞的增殖、侵袭和迁移能力均低于空白对照组和阴性对照组,Fat-1组细胞凋亡水平高于空白对照组和阴性对照组(P均<0.01)。Fat-1组细胞中p-PI3K、p-Akt和p-mTOR表达水平均低于空白对照组和阴性对照组(P<0.01)。结论:Fat-1基因可通过增加细胞内ω-3PUFA含量,靶向作用于PI3K/Akt/mTOR通路,抑制宫颈癌Hela细胞的增殖、侵袭和迁移,促进细胞凋亡。

关键词(KeyWords)： Fat-1基因;宫颈癌;ω-3多不饱和脂肪酸;PI3K/Akt/mTOR通路

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(No:2016A020215115);广东省科技计划项目(No:2017ZC0089);; 广州市天河区医疗卫生专项重点项目(No:201604KW010)

作者(Authors): 张军;蒋惠萍;陈咏宁;王丽婧;刘春花;郑曰燕;龚时鹏;郭遂群;

DOI: 10.13283/j.cnki.xdfckjz.2019.04.001

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