马宁;邱丽华;

• 1:上海交通大学医学院附属仁济医院妇产科上海市妇科肿瘤重点实验室

摘要(Abstract)：

目的:探讨肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)在调控上皮性卵巢癌(EOC)细胞对顺铂(DDP)敏感性中发挥的作用。方法:不同浓度TWEAK刺激A2780/cDDP细胞后,采用CCK-8法检测细胞的增殖能力及DDP半数抑制浓度(IC50)的变化;采用流式细胞术检测细胞凋亡率的变化;采用透射电镜检测细胞核染色质形态和凋亡小体的变化;Western blot法检测细胞凋亡相关蛋白caspase 3的活化情况。结果:TWEAK对A2780/cDDP细胞的增殖能力无影响,但能显著增加A2780/cDDP细胞对DDP的敏感性。流式结果显示,随着TWEAK剂量增加,A2780/cDDP细胞的凋亡显著增加。TWEAK刺激后A2780/cDDP细胞中caspase 3活化体表达增加,细胞核染色质发生形态变化,诱导了细胞的凋亡及凋亡小体形成。结论:TWEAK通过活化凋亡相关通路,促进EOC细胞凋亡,提高了DDP耐药EOC细胞对DDP的敏感性。这可能为临床开创逆转EOC铂类耐药的治疗新途径提供理论依据。

关键词(KeyWords)： 卵巢肿瘤;TWEAK;耐药;凋亡

Abstract:

Keywords:

基金项目(Foundation): 上海市教委重点学科;; 国家自然基金项目(No:81272884)

作者(Authors): 马宁;邱丽华;

DOI: 10.13283/j.cnki.xdfckjz.2013.12.003

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