三苯氧胺对人子宫内膜癌细胞系Ishikawa细胞增殖的影响Effect of tamoxifen on proliferation of human endometrial carcinoma cell line Ishikawa cells
王敏;刘昆;惠京;
摘要(Abstract)：
目的:探讨三苯氧胺(TAM)在体外对Ishikawa人子宫内膜癌细胞株增殖的影响及其作用机制。方法:采用MTT法观察不同浓度TAM作用不同时间对Ishikawa细胞增殖的影响,另应用MTT法、流式细胞术、免疫细胞化学方法观察比较TAM、17β-雌二醇(17β-E2)及两者联合作用不同时间后对Ishikawa细胞增殖率,细胞周期时相分布以及C-myc、bcl-2、Bax 3种蛋白表达的影响。结果:TAM对Ishikawa细胞的促增殖作用在一定剂量范围内有浓度依赖性,可使G0/G1期细胞比例下降,S期细胞比例升高;C-myc、bcl-2蛋白表达增加,Bax蛋白表达下降。结论:体外TAM可能通过调节细胞周期时相分布及上调C-myc蛋白、bcl-2蛋白,下调Bax蛋白表达,促进Ishikawa人子宫内膜癌细胞增殖。
关键词(KeyWords)： 三苯氧胺;17β-雌二醇;子宫内膜;C-myc肿瘤基因;基因bcl-2;Bax
基金项目(Foundation):
作者(Authors): 王敏;刘昆;惠京;
DOI: 10.13283/j.cnki.xdfckjz.2010.10.019
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