章燕;祝亚平;鲍伟;

• 1:上海交通大学医学院附属第一人民医院妇产科

摘要(Abstract)：

目的:探讨下调microRNA-107(miR-107)表达对人子宫内膜样腺癌细胞系Ishikawa增殖迁移和侵袭能力的影响及可能机制。方法:将miR-107抑制剂(miR-107 inhibitor)瞬时转染Ishikawa细胞,下调miR-107表达。实时荧光定量PCR法(qRT-PCR)检测miR-107表达,CCK-8法检测细胞增殖能力,细胞划痕方法检测细胞迁移能力,Transwell小室法检测细胞侵袭能力,Western blot法检测ER-α受体蛋白表达水平。结果:转染miR-107 inhibitor的Ishikawa细胞中miR-107表达水平显著降低,细胞的增殖迁移和侵袭能力降低,细胞中ER-a受体蛋白表达水平增高。结论:下调miR-107表达可能通过上调ER-α表达抑制Ishikawa细胞的增殖迁移和侵袭能力。

关键词(KeyWords)： 子宫内膜样腺癌;微小RNA;miR-107;Ishikawa细胞;增殖;迁移;侵袭;ER-α

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No:81372795)

作者(Authors): 章燕;祝亚平;鲍伟;

DOI: 10.13283/j.cnki.xdfckjz.2018.05.003

参考文献(References)：

• [1]Siegel R,Ma JM,Zou ZH,et al.Cancer statistics,2014[J].CA Cancer J Clin,2014,64(1):9-29
• [2]Dedes KJ,Wetterskog D,Ashworth A,et al.Emerging therapeutic targets in endometrial cancer[J].Nat Rev Clin Oncol,2011,8(5):261-271
• [3]Bansal N,Yendluri V,Wenham RM.The molecular biology of endometrial cancers and the implications for pathogenesis,classification,and targeted therapies[J].Cancer Control,2009,16(1):8-13
• [4]Lee RC,Feinbaum RL,Ambros V.The C.elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J].Cell,1993,75(5):843-854
• [5]吴瑕,何君,王文军.miR-107靶向NID2通过Notch信号通路调控肺癌细胞的增殖侵袭[J].中国免疫学杂志,2017,33(11):1662-1667
• [6]Chung TK,Cheung TH,Huen NY,et al.Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women[J].Int J Cancer,2009,124(6):1358-1365
• [7]苏宁,陈一飞,严沁,等.下调microRNA-205表达对人子宫内膜癌Ishikawa细胞增殖和侵袭的作用[J].现代妇产科进展,2012,21(7):520-524
• [8]Chen S,Sun KX,Liu BL,et al.MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-alpha[J].Mol Cancer,2016,15(1):11
• [9]Liu BL,Sun KX,Zong ZH,et al.MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C(Rho C)[J].Oncotarget,2016,7(6):6649-6664
• [10]Xu YY,Tian J,Hao Q,et al.MicroRNA-495 downregulates FOXC1 expression to suppress cell growth and migration in endometrial cancer[J].Tumour Biol,2016,37(1):239-251
• [11]Jiang FZ,Liu T,He YY,et al.MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo[J].BMC Cancer,2011,11(1):425
• [12]Chen PS,Su JL,Cha ST,et al.miR-107 promotes tumor progression by targeting the let-7 microRNA in mice and humans[J].J Clin Invest,2017,127(3):1116
• [13]Li XH,Zhang Y,Shi YQ,et al.MicroRNA-107,an oncogene microRNA that regulates tumour invasion and metastasis by targeting DICER1 in gastric cancer[J].J Cell Mol Med,2011,15(9):1887-1895
• [14]Takahashi Y,Forrest AR,Maeno E,et al.MiR-107 and MiR-185 can induce cell cycle arrest in human nonsmall cell lung cancer cell lines[J].PLo S One,2009,4(8):e6677
• [15]Wang WX,Wilfred BR,Madathil SK,et al.miR-107 regulates granulin/progranulin with implications for traumatic brain injury and neurodegenerative disease[J].Am J Pathol,2010,177(1):334-345
• [16]Datta J,Smith A,Lang JC,et al.microRNA-107 functions as a candidate tumor-suppressor gene in head and neck squamous cell carcinoma by downregulation of protein kinase Cvarepsilon[J].Oncogene,2012,31(36):4045-4053
• [17]Backes FJ,Walker CJ,Goodfellow PJ,et al.Estrogen receptor-alpha as a predictive biomarker in endometrioid endometrial cancer[J].Gynecol Oncol,2016,141(2):312-317
• [18]Lopez-Romero R,Garrido-Guerrero E,Rangel-Lopez A,et al.The cervical malignant cells display a down regulation of ER-alpha but retain the ER-beta expression[J].Int J Clin Exp Pathol,2013,6(8):1594-1602
• [19]Zhai Y,Bommer GT,Feng Y,et al.Loss of estrogen recepter I enhances cervical cancer invasion[J].Am J Pathol,2010,177(2):884-895