地塞米松体外诱导宫颈癌细胞株凋亡及其机制的研究Mechanism of apoptosis of HeLa cells induced by dexamethasone in vitro
徐波,顾美礼,卞度宏
摘要(Abstract):
目的 :研究地塞米松 (Dex)体外能否诱导宫颈癌细胞株 (HeLa细胞 )发生凋亡及其机制。方法 :采用透射电镜、DNALadder、流式细胞仪等方法进行凋亡检测 ,用ECLWesternblot方法检测凋亡过程中相关基因表达的变化。结果 :地塞米松体外作用于HeLa细胞后 ,透射电镜观察到了凋亡小体 ;DNALadder法也检测到了凋亡时DNA降解形成的梯带 ;流式细胞仪检测到了细胞凋亡峰 ,且峰值随着处理时间的延长而增高。在凋亡过程中 ,凋亡相关基因bcl 2表达下调 ,而p5 3基因表达无明显改变。结论 :地塞米松体外能诱导HeLa细胞发生凋亡 ,其机制与bcl 2基因的表达下调有关
关键词(KeyWords): 宫颈肿瘤;细胞凋亡;HeLa细胞;地塞米松;基因,bcl-2
基金项目(Foundation):
作者(Author): 徐波,顾美礼,卞度宏
DOI: 10.13283/j.cnki.xdfckjz.2003.03.007
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