钱孟玲;陈素琴;刘宏;

• 1:河北医科大学第二医院妇产科

摘要(Abstract)：

目的:探讨PAI-1基因启动子区4G/5G和TGFβ1基因-509C/T基因多态性与中国河北省汉族育龄妇女Ⅲ、Ⅳ期EMs遗传易感性的关系。方法:用病例对照研究法,75例Ⅲ、Ⅳ期EMs患者与82例对照组的外周血白细胞为样本,用PCR-RFLP技术分析PAI-1基因启动子区4G/5G和TGFβ1基因-509C/T基因多态性分布频率。结果:PAI-1基因-6754G/5G的3种基因型:4G/4G、4G/5G、5G/5G在EMs组和对照组的分布频率分别为:69.3%,28.0%,2.7%;12.2%,31.7%,56.1%。4G/5G等位基因在两组的分布频率为83.3%,16.7%;28.1%,71.9%。两组差异有统计学意义(P<0.05)。TGFβ1基因-509C/T3种基因型:CC/CT/TT在EMs和对照组的分布频率分别为:10.7%,58.7%,30.7%;57.3%,41.5%,1.2%。C/T等位基因在两组的分布频率为40%,60%;78%,22%。两组差异有统计学意义(P<0.05)。结论:携带PAI-1基因启动子区-6754G等位基因增加了患Ⅲ、Ⅳ期EMs的危险性。携带TGFβ1基因-509T等位基因患Ⅲ、Ⅳ期EMs的危险性增加。

关键词(KeyWords)： 子宫内膜异位症;基因多态性;聚合酶链反应;限制性片段长度多态性;纤溶酶原激活物抑制物-1;转化生长因子β1

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 钱孟玲;陈素琴;刘宏;

DOI: 10.13283/j.cnki.xdfckjz.2010.03.018

参考文献(References)：

• [1]Bruse C,Guan Y,Carlberg M,et al.Basal release of uro-kinase plasminogen activator,plasminogen activator inhib-itor-1,and soluble plasminogen activator receptor from separated and cultured endometriotic and endometrial stromal and epithelial cells[J].Fertil Steril,2005,83:1155-1160
• [2]Diamanti-Kandarakis E,Palioniko G,Alexandraki K,et a-l.The prevalence of4G/5G polymorphism of plasminogen activator inhibitor-1(PAI-1)gene in polycystic ovarian syndrome and its association with plasma PAI-1levels[J].Eur J Endocrinol,2004,150:793-798
• [3]Bruse C,Radu D,Bergqvist A.In situ localization of mR-NA for the fibrinolytic factors uPA,PAI-1and uPAR in endometriotic and endometrial tissue[J].Mol Hum Re-prod,2004,10:159-166
• [4]Gilabert-Estelles J,Estelles A,Gilabert J,et al.Expres-sion of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis[J].Hum Reprod,2003,18:1516-1522
• [5]Guan YM,Carlberg M,Bruse C,et al.Effects of hormones on uPA,PAI-1and suPAR from cultured endometrial and ovarian endometriotic stromal cells[J].Acta Obstet Gyne-col Scand,2002,81:389-397
• [6]Hellebrekers BW,Emeis JJ,Kooistra T,et al.A role for the fibrinolytic system in postsurgical adhesion formation[J].Fertil Steril,2005,83:122-129
• [7]Mohamed A,Edward J,Pand Robert F,et al.Genetic pol-ymorphism in the fibrinolytic system and endometriosis[J].Obstet Gynecol,2006,108:163-168
• [8]Komiyama S,Aoki D,Komiyama M,et al.Local activation of TGF-beta1at endometriosis sites[J].J Reprod Med,2007,52:306-312
• [9]Koks CA,Demir-Weusten AY,Groothuis PQ,et al.Men-struum induces changes in mesothelial cell morphology[J].Gynecol Obstet Invest,2000,50:13-18
• [10]Witz CA,Allsup KT,Montoya R,et al.Culture of men-strual endometrium with peritoneal explants and meso-thelial monolayers confirms attachment to intact mesothe-lial cells[J].Hum Reprod,2002,17:2832-2838
• [11]Witz CA,Cho S,Centonze VE,et al.Time series analysis of transmesothelial invasion by endometrial stromal and epithelial cells using three-dimensional confocal micros-copy[J].Fertil Steril,2003,79:770-778
• [12]Komiyama S,Aoki D,Komiyama M,et al.Local activa-tion of TGF-beta1at endometriosis sites[J].J Reprod Med,2007,52:306-312
• [13]Hsieh YY,Chang CC,Tsai FJ,et al.Polymorphism for transforming growth factor beta1-509(TGF-β1-509):association with endometriosis[J].Biochem Genet,2005,43:203-210