宫颈上皮内瘤变及宫颈癌患者外周血Treg/Th17细胞及相关细胞因子的检测与临床意义The Treg/Th17 imbalance in patients with cervical cancer and cervical intraepithelial neoplasia
熊苗;陆继红;舒慧敏;赵爱民;史桂英;杨宾烈;
摘要(Abstract)：
目的:探讨Treg/Th17平衡失调在宫颈上皮内瘤变(CIN)及宫颈癌病情进展中的作用及其参与CIN及宫颈癌免疫逃避的具体机制。方法:研究对象分为3组:宫颈癌组(34例),CIN组(47组),对照组(30例)。采用流式细胞术检测CIN及宫颈癌患者外周血单个核细胞(PBMC)中Th17细胞及Treg细胞的表达频率,采用酶联免疫吸附试验(ELISA)方法检测相关细胞因子(IL-23、IL-10、IL-17、IL-6、TGF-β)的浓度。结果:与对照组相比,Treg细胞及其相关细胞因子(TGF-β、IL-10)在CIN及宫颈癌患者外周血中的表达均明显增高(P<0.05),且宫颈癌组的表达亦明显高于CIN组(P<0.05)。与对照组相比,Th17细胞及其相关细胞因子(IL-23、IL-17、IL-6)在CIN及宫颈癌患者外周血中的表达均明显降低(P<0.05),且宫颈癌组的表达亦明显低于CIN组(P<0.05)。结论:随着CIN向宫颈癌的进展,Treg/Th17平衡向Treg细胞偏离,这一失衡参与了CIN的进展及宫颈癌的免疫逃避,其具体机制可能与二者在肿瘤免疫内环境相关的细胞因子调节有关。
关键词(KeyWords)： 宫颈肿瘤;宫颈上皮内瘤变;T淋巴细胞;抗原,CD4;抗原,CD25;Th17细胞;CD4+CD25+调节性T细胞
基金项目(Foundation): 上海市浦东新区科委资助项目(No:PKJ2009-Y-25)
作者(Authors): 熊苗;陆继红;舒慧敏;赵爱民;史桂英;杨宾烈;
DOI: 10.13283/j.cnki.xdfckjz.2012.06.016
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