胰岛素样生长因子-II对卵巢癌细胞SKOV3中MMP-9表达的调节作用Effect of IGF-II stimulation on the expression of matrix metalloproteinase-9 in human ovarian cancer cell line SKOV3
吴小华,王利红,宋俊芬,王伟萍,单保恩
摘要(Abstract)：
目的 :探讨胰岛素样生长因子 II(IGF II)对卵巢癌细胞SKOV3的基质金属蛋白酶 9(MMP 9)、金属蛋白酶组织抑制剂 1(TIMP 1)表达的影响。方法 :细胞 (1.5× 10 5/ml)培养 4 8h后 ,加入不同浓度的IGF II ,再培养 18h。用RT PCR法测定MMP 9mRNA、TIMP 1mRNA水平 ,用明胶酶谱法半定量测定MMP 9蛋白水平的变化。结果 :IGF II可刺激MMP 9蛋白的分泌。随着IGF II浓度增加 ,诱导作用增强 ;浓度较高时 ,MMP 9的分泌量轻度下降。但IGF II不影响MMP 9和TIMP 1的基因表达水平。结论 :IGF II可刺激卵巢癌细胞SKOV3中MMP 9的分泌 ,在卵巢癌的侵袭转移方面可能起重要作用
关键词(KeyWords)： 胰岛素样生长因子II;卵巢肿瘤;细胞系;基质金属蛋白酶;金属蛋白酶组织抑制剂-1;聚合酶链反应
基金项目(Foundation): 河北省自然科学基金资助项目(项目编号 30 1391)
作者(Author): 吴小华,王利红,宋俊芬,王伟萍,单保恩
DOI: 10.13283/j.cnki.xdfckjz.2003.06.006
参考文献(References)：
- [1] DavidsonB ,GoldbergI,GotliebWH ,etal.HighlevelsofMMP 2,MMP 9,MT MMPandTIMP 2mRNAcorrelatewithpoorsurvivalinovariancarcinoma[J].ClinicExpMet,1999,17:799808
- [2] ConoverCA ,HartmannLC ,BradleyS ,etal.Biologicalchar acterizationofhumanepithelialovariancarcinomacellsinpri maryculture:theinsulin likegrowthfactorsystem[J].ExpCellRes,1998,238:439449
- [3] 蔡广研,陈香美,傅博,等.纤维蛋白诱导活化系膜细胞表达明胶酶A、B的机制研究[J].解放军医学杂志,2001,26:877879
- [4] 路艳艳,周柔丽.整合素α6β1增强人肝癌细胞侵袭性[J].中华肿瘤杂志,2000,22:287289
- [5] MiraE ,ManesS ,LacalleRA ,etal.Insulin likegrowthfactorI triggeredcellmigrationandinvasionaremediatedbymatrixmetalloproteinase9[J].Endocrinology,1999,140:16571664
- [6] AkataK ,ShigemasaK ,NagaiN ,etal.Expressionofmatrixmetalloproteinases(MMP 2,MMP 9,MT1MMP)andtheirinhibitors(TIMP 1,TIMP 2)incommonepithelialtumorsoftheovary[J].IntJOncol,2000,17:673681
- [7] MartinDC ,FowlkesJL ,BabicB ,etal.Insulin likegrowthfactorIIsignalinginneoplasticproliferationisblockedbytransgenicexpressionofthemetalloproteinaseinhibitorTIMP 1[J].JCellBiol,1999,146:881892
- [8] LeeO ,BaeSK ,LeeMH ,etal.Identificationofangiogenicpropertiesofinsulin likegrowthfactorinvitroangiogenesismodels[J].BrJCancer,2000,82:385391
- [9] CanalisE ,RydzielS ,DelanyAM ,etal.Insulin likegrowthfactorsinhibitinterstitialcollagenasesynthesisinbonecellcultures[J].Endocrinology,1995,136:13481354