HeLa细胞中抑癌基因-间隙连接基因Cx43及其蛋白产物表达缺失Expression of antioncogene connexin43 mRNA and its protien in human cervical carcinoma cell line HeLa
马向东,陈必良,王德堂,辛晓燕
摘要(Abstract)：
目的：探讨新一代肿瘤抑制基因-间隙连接基因Cx43及其蛋白产物在人子宫颈癌细胞系HeLa中的表达及意义。方法：应用核酸分子原位杂交和流式细胞仪分析技术，研究人子宫颈癌细胞系HeLa及阳性对照细胞人正常肝细胞系QZG中，间隙连接基因Cx43mRNA及其蛋白产物的表达规律。结果：原位杂交显示，Cx43mRNA在HeLa细胞中呈阴性，在QZG细胞中呈强阳性。流式细胞仪分析示Cx43蛋白在HeLa、QZG细胞中阳性细胞计数率分别为1．9％和99．0％，差异有高度显著性（P＜0．01）。结论：CX43基因及其蛋白产物在HeLa中表达缺失可能与子宫颈癌发生恶性表型相关。
关键词(KeyWords)： 间隙连接;子宫肿瘤;杂交;流式细胞术;细胞;培养
基金项目(Foundation):
作者(Author): 马向东,陈必良,王德堂,辛晓燕
DOI: 10.13283/j.cnki.xdfckjz.1999.04.005
参考文献(References)：
- 1. Steinberg TH. Gap junction function the messenger and the message. Am J Pathol, 1998; 152 (4): 851
- 2' Kumar NN, Gilula NB. The gap junction communication channel. Cell, 1996; 84: 381
- 3. Holder JW,Elmope E,Barrett JC. Gap junction function and cancer. Cancer Res, 1993; 53: 3475
- 4. Mesnil M, Krutovskikh V, Piccoli C, et al. Negative growth control of HeLa cells by connexin speciesspecificity. Cancer Res, 1995; 55: 629
- 5. Toyofuku T,Yabuki M, Otsu K, et al. Intercellular calcium signaling via gap junction in connexin-43-transfected cells. J Biol Chem, 1998; 273 (3): 1519
- 6. Zhu W, Mironov N, Yamasaki H. Increased genetic stability of HeLa cells after connexin 43 genetransfection. Cancer Res, 1997; 57: 2148
- 7. Fick J,Barker FG,Dazin P,et al. The extent of heterocellular communication mediated by gap junctionsis predictive of bystander tumor cytotoxicity in vltro. Pro Natl Acad Sci USA, 1995; 92: 11071
- 8. Dilber MS, Abedi MR, Christensson M, et al. Gap junctions promote the bystander effect of herpessimplex virus thymidine kinase in vivo. Cancer Res, 1997; 57: 1523
- 9. Nesnil M,Piccoli C,Tiraby G,et al. Bystander killing of cancer cells by simplex virus thymidine kinasegene is mediated by connexins. Pro Natl Acad Sci USA, 1996 193: 1831